MCQs for Up Bsc Nursing CNET ABVMU Biotechnology : Principles and Process
1. Which of the following is a process used in biotechnology to transfer genes into a host organism?
a) PCR (Polymerase Chain Reaction)
b) Gel Electrophoresis
c) Cloning
d) Recombinant DNA technology
2. The enzyme responsible for cutting DNA at specific sequences in recombinant DNA technology is called:
a) DNA ligase
b) DNA polymerase
c) Restriction enzyme
d) RNA polymerase
3. What is the function of a selectable marker in recombinant DNA technology?
a) To differentiate between transformed and non-transformed cells
b) To help in DNA replication
c) To amplify the DNA
d) To join DNA fragments together
4. The process of separating DNA fragments based on their size and charge using an electric field is called:
a) PCR (Polymerase Chain Reaction)
b) DNA sequencing
c) Gel electrophoresis
d) DNA replication
5. The enzyme used to produce complementary DNA (cDNA) from an mRNA template is:
a) DNA polymerase
b) Reverse transcriptase
c) DNA ligase
d) Restriction enzyme
6. The technique used to amplify a specific region of DNA to produce multiple copies is called:
a) Recombinant DNA technology
b) DNA sequencing
c) PCR (Polymerase Chain Reaction)
d) Gel electrophoresis
7. Which restriction site is not present in pBR322 plasmid?
a) EcoRI
b) Hind III
c) Hind II
d) Sal I
8. pBR322 plasmid is resistant to which of the following antibiotics?
a) Ampicillin
b) tetracycline
c) Penicillin
d) both a and b
9. Which DNA fragment is the smallest?
a) A
b) B
c) C
d) D
10. Which of these is the feature of a cloning vector?
a) Ori site
b) Selectable marker
c) Cloning site
d) All of the above
Answer Key
1. d) Recombinant DNA technology
2. c) Restriction enzyme
3. a) To differentiate between transformed and non-transformed cells
4. c) Gel electrophoresis
5. b) Reverse transcriptase
6. c) PCR (Polymerase Chain Reaction)
7. c) Hind II
8. d) both a and b
9. d) D
10. d) All of the above
| Chapter Name | Chapter Link |
|---|---|
| Sexual Reproduction in Flowering Plants | Link |
| Human Reproduction | Link |
| Reproductive Health | Link |
| Principles of Inheritance and Variation | Link |
| Molecular Basis Of Inheritance | Link |
| Evolution | Link |
| Human Health and Disease | Link |
| Microbes in Human Welfare | Link |
| Biotechnology : Principles and Process | Link |
| Biotechnology and its Applications | Link |
| Organism And Population | Link |
| Ecosystem | Link |
| Biodiversity and Conservation | Link |
